Discovery has been rolled out to the launch pad to await its April launch to ISS as STS-131. The launch window is as follows:
| 5Apr10 | 0616-0629 EDT |
| 6Apr10 | 0553-0603 EDT |
| 7Apr10 | 0528-0541 EDT |
| 8Apr10 | 0505-0515 EDT |
| 9Apr10 | 0444-0453 EST |
| 10Apr10 | 0417-0427 EST |
| 11Apr10 | 0356-0404 EST |
| 12Apr10 | 0329-0339 EST |
| 13Apr10 | 0308-0316 EST |
| 14Apr10 | 0249-0250 EST |
| Daylight Launch after the start of civil twilight | |
| Twilight Launch astronomical twilight through nautical twilight | |
| Night Launch before the start of astronomical twilight | |
| 7Apr10 | Rendesvous, RPM, Docking |
| 15Apr10 | Undocking, Flyaround, Separation |
| 17Apr10 | Deorbit Burn |
| 18Apr10 | Landing at KSC |
